Organisms are built of a large number of diverse and highly specialized cells. To better understand the roles of each cell, we should study them at the single-cell level. One of the most significant achievements of analytical sciences in the last ten years was implementing a droplet microfluidic technology for analyzing single cells using RNA sequencing (RNA-seq). Droplet-based profiling of single-cell transcriptomes allowed for a very detailed description of the physiological state of thousands of single cells and led to an efficient determination of cell types, trajectories, and interactions. These methods are especially indispensable in research fields dealing with highly heterogeneous tissues – such as oncology, immunology, embryology, and other areas of biomedical research. In our research projects, we are applying already established unique techniques, as well as developing novel multi-step microdroplet assays to study transcriptomes and genomes of single cells.
