Grant number: 2015/19/N/NZ9/00478
The objectives of this project are to investigate the effect of addition of zeolites on changes in the structure of microbial community composition during anaerobic digestion of sewage sludge and to track the process of zeolite colonization by biogas-community. Various types of natural and synthetic zeolites will be added and tested in anaerobic digestion process conducted in laboratory reactor. The effect of different type zeolites on kinetic of anaerobic digestion of sewage sludge process and will be estimated, basing on the comprehensive analyzes of biogas yield and physico-chemical parameters. Analysis of community composition will be done at the metagenomic DNA level by the sequencing of marker gene 16S rRNA of Bacteria and Archaea. The rate of zeolite’s colonization will be also searched with the use of scanning electron microscopy analyzes. Research conducted during this project allow to: (i) estimate and compare impact of different types of zeolites on the rate and efficiency of anaerobic digestion of sewage sludge, (ii) correlate the physiological effect of zeolites addition with changes in the structure of microbial community, (iii) explain the interaction between zeolites and microorganisms, especially in the context of zeolite’s colonization by microorganisms.